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KMID : 1134820090380050606
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Journal of the Korean Society of Food Science and Nutrition
2009 Volume.38 No. 5 p.606 ~ p.611
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Comparison of Four Different Isolation Media for Staphylococcus aureus
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Oh Min-Hee
Kang Seong-Il
Hong Sang-Pil
Oh Se-Wook
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Abstract
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Performance test was carried out between selective media which are generally used in Staphylococcus aureus isolation from food. Sensitivity, determined according to the appearance of characteristic colonies when 30 different S. aureus strains were tested, resulted as Baird-Parker agar (RPF)> PetrifilmTM Staph Express plate> Baird-Parker agar> Mannitol salt agar. Also, the four different media showed the same selectivity because all tested media did not produce the false positive colonies. Recovery efficiency from the artificially inoculated foodstuff was almost the same for the tested media. Presumptive colonies were collected from the dried fishery product using Mannitol salt agar and collected strains were tested on 4 different selective agar. Almost presumptive strains did not show the false positive colonies except for S. carnosus ssp carnosus. This strain was identified as false positive colonies on Mannitol salt agar, Baird-Parker agar and PetrifilmTM Staph Express plate. But Baird-Parker agar (RPF) did not show the false positive colonies with the same strains. So, it was concluded that the Baird-Parker agar (RPF) has more higher selectivity than other tested media in this experiment.
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KEYWORD
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Staphylococcus aureus, media, isolation, comparison, specificity
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